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. 2015 Jul 1;19(8):2019–2031. doi: 10.1111/jcmm.12599

Figure 6.

Figure 6

Effect of EtxB on development of DC in bone marrow cultures supplemented with Flt3L. Bone marrow cells were treated to lyse red blood cells and cultured (2 × 106 cells/ml) in 200 ng/ml Flt3L for 8 days to induce DC development. The total cell population was then collected and incubated for 24 hrs with either EtxB (10 μg/ml), LPS (10 ng/ml), a combination of EtxB and LPS, or medium as a control. Cells were then collected and stained for CD11c and SIRPα to detect the CD11c+ SIRPα subset containing pDC and CD8− cDC, and the CD11c+ SIRPα− subset containing CD8+ cDC. Cells were stained for detection of the activation markers CD80, CD86 and MHC-II by flow cytometry. Isotype control antibodies were used to set gates. MHC-II expression was measured in terms of MFI, and expression of CD80 and CD86 as % cells staining. Data are shown as mean ± SE of duplicates represented as crosshairs. Significant differences between pairs at P ≤ 0.05, P ≤ 0.01 and P ≤ 0.001 are shown by *, ** and *** respectively.