Fig 5. Y. pestis Biofilm Production is not Influenced by Exogenous cAMP.

(A) Relative biofilm production of KIM6+ and KIM6+Δcrp when grown in BCS medium supplemented with 0.2% glucose containing either 3 mM cAMP prepared in K-phosphate buffer or an equal volume of plain K-phosphate buffer. (B) Relative biofilm production of CO92 and CO92Δcrp when grown in BCS medium supplemented with 0.2% glucose containing either 3 mM cAMP prepared in K-phosphate buffer or an equal volume of plain K-phosphate buffer. Identical source inoculum per strain was utilized for each media type. Error bars reflect standard deviation from the mean derived from two independent experiments, each consisting of 6 technical replicates. No statistically significant change in crystal violet absorption as determined by one-way ANOVA was calculated for both the crp-deficient mutants and the respective parental controls when cultured in media containing 3mM cAMP or an equal volume of K-phosphate buffer.