Table 1. Bacterial Strains and Plasmids.
| Y. pestis strains | Characteristics* | Reference |
| CO92 | Pgm+ pCD+ | [48] |
| CO92 pgm- | Pgm- pCD+ | Motin collection |
| CO92Δcrp | Scarless deletion of the crp gene | This study |
| CO92ΔcsrA 5a | Scarless deletion of the csrA gene clone 5a | This study |
| CO92ΔcsrA 3b | Scarless deletion of the csrA gene clone 3b | This study |
| CO92ΔcsrA csrA’ 5a | Scarless Restoration of the csrA gene of clone 5a | This study |
| CO92ΔcsrA csrA’ 3b | Scarless Restoration of the csrA gene of clone 3b | This study |
| CO92ΔglgCAP | Scarless deletion of the glgCAP gene | This study |
| CO92ΔcsrA ΔglgCAP | Scarless deletion of the glgCAP gene from CO92ΔcsrA 5a | This study |
| CO92ΔhmsP | Scarless deletion of the hmsP gene | This study |
| CO92ΔcsrA ΔhmsP | Scarless deletion of the hmsP gene from CO92ΔcsrA 5a | This study |
| KIM6+ | Pgm+ pCD− | Brubaker collection |
| KIM6+ pgm- | Pgm- pCD− | Motin collection |
| KIM6+Δcrp | Scarless deletion of the crp gene | This study |
| KIM6+ΔcsrA 2:14 | Scarless deletion of the csrA gene clone 2:14 | This study |
| KIM6+ΔcsrA 4:12 | Scarless deletion of the csrA gene clone 4:12 | This study |
| KIM6+ΔcsrA csrA’ 2:6 | Scarless restoration of the csrA gene in clone 2:14 | This study |
| E. coli strains | Characteristics | Reference |
| DH5α | F- φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) gal- phoA supE44 λ- thi-1 gyrA96 relA1 | Invitrogen (Carlsbad, CA) |
| Plasmids | Characteristics | Reference |
| pKD46 | Mutagenesis helper plasmid, source of Lambda Red Recombinase, ApR | [31] |
| pKD4_Km-sacB | Cloned sacB from pCVD442 in NgoMIV site of pKD4, source of kan-sacB cassette, KmR, ApR, SucS | [33] |
| pBluescript SK+ | Cloning vector, ApR | Stratagene (La Jolla, CA) |
| pBluescript/crp | Cloned crp gene derived from KIM6+ into pBluescript SK+, ApR | This study |
* All Y. pestis strains contained the pMT1 and pPCP plasmids.