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. 2015 Aug 25;11(8):e1005485. doi: 10.1371/journal.pgen.1005485

Fig 2. Essential function of CCNYL1 for male fertility and sperm motility.

Fig 2

(A) Measurement of CCNYL1 or CCNY protein expression in testes of WT, Ccnyl1-/-, and Ccny-/- mice, with β-actin serving as loading control. (B) Male fertility of adult WT, Ccnyl1-/- and Ccny-/- mice (n ≥ 6 per group). Pregnancy was counted in mating cages with male WT, Ccnyl1-/-, or Ccny-/- mice. (C) Number of spermatozoa in male WT, Ccnyl1-/-, and Ccny-/- mice collected from the caput and cauda epididymidis (n ≥ 6 per group). (D) HE staining of testicular sections from adult WT and Ccnyl1-/- mice. Scale bar: 100μm. (E-G) The percentage of total and progressive motility (E), velocity (F) and the motility distribution (G) of spermatozoa were measured by CASA (n = 5 animals per group). (H) In vitro fertilization assay. Oocytes were incubated with cauda epididymidal spermatozoa collected from WT or Ccnyl1-/- mice. The development of the oocytes was monitored in vitro. Data in bar graphs are presented as mean ± SEM. *P < 0.05; **P < 0.01.