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Time Considerations for Select Protocols
| PROTOCOL | DESCRIPTION | TIME REQUIRED |
|---|---|---|
| Basic Protocol 1 or 2 | Gonococcal transformation | 4–5 days |
| Basic Protocol 3 | Electroporation | 4 days |
| Basic Protocol 4 | Gonococcal mutagenesis using selection and counter selection | 16–20 days |
| Basic Protocol 5 | Shuttle mutagenesis | 12–14 days |
| Basic Protocol 6 | Chemical mutagenesis | 2 days |
| Basic Protocol 7 or Alternate Protocol 2 | Complementation | 4–5 days |
| Basic Protocol 8 | Chromosome preparation | 2 days |
