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| glucose | 10.0 |
| ammonium bicarbonate | 2.0 |
| sodium acetate | 10 |
| glutamine | 0.75 |
| spermidine& | 0.2 |
| arginine | 0.1 |
| hypoxanthine | 0.05* |
| uracil | 0.05* |
| oxaloacetate | 0.05 |
| thiamine HCl | 0.05 |
| NAD | 0.01 |
| ornithine | 0.01 |
| sodium lactate(60% syrup) | 2.5ml |
Adjust pH to 6.8 with 1M NaOH and sterilize through 0.22μ filter.
Dissolve in 5ml 1M NaOH.
Spermidine is not necessary for many N. gonorrhoeae strains.
This medium can be stored at 4 C for up to three weeks.
