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Protein expression of key insulin signaling components in the gastrocnemius muscle at 16 weeks. Protein levels of Irs-1, Akt2 and Glut4 in the gastrocnemius of rats from NL and SL were assessed by western blotting. Representative blots are shown in (a). Relative protein levels were quantified by scanning densitometry of the bands (b). Results are expressed as mean ± S.E.M (n = 6, 4 litters from each group). ** P < 0.01
