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CD40-pre-activated CLL cells were cultured for 36 h in the presence or absence of IL21, and their conditioned media were tested for chemokine concentrations by ELISA. IL21 inhibited the secretion of CCL22, CCL17, CCL4 and CCL3 from CLL cells in different patients (no. 13), while it up-regulated CXCL9 and CXCL10 secretion. p values were obtained using two-tailed paired t-test.
