Table 2. Gene-expression in IL21 treated vs untreated CLL and normal B-cells, as assessed by RT-qPCR.

Gene	Arrays 1		Technical validation by RT-qPCR 1		Independent validation by RT-qPCR 2		Normal B cells by RT-qPCR 3
Gene symbol	Module	Fold change*	Fold change*	p-value	Fold change*	p-value	Fold change*	p-value
CCL17	MEpink	0.19 ↓↓	0.08 ↓↓	0.00019	0.1 ↓↓	0.000136	0.14 ↓↓	0.000538
CCL22	MEpink	0.35 ↓↓	0.21 ↓↓	0.00167	0.13 ↓↓	0.0000026	0.36 ↓↓	0.00226
CD40	MEpink	0.66 ↓	0.53 ↓	0.000136	0.51 ↓	0.00812	0.5	0.118
DDR1	MEpink	0.44 ↓↓	0.27 ↓↓	3.53E-05	0.39 ↓↓	0.000558	0.46	0.9
EBI3	MEpink	0.52 ↓	0.4 ↓↓	0.000153	0.36 ↓↓	0.000108	0.73 ↓	0.0114
ITPKB	MEpink	0.61 ↓	0.54 ↓	0.00606	0.74 ↓	0.0348	0.74 ↓	0.0348
PIK3CD	MEpink	0.59 ↓	0.42 ↓↓	0.000602	0.43 ↓↓	0.000312	0.52 ↓	0.00791
TNF	MEpink	0.61 ↓	0.44 ↓↓	0.0009631	0.57 ↓	0.067	1.11	0.731
TNFSF4	MEpink	0.4 ↓↓	0.34 ↓↓	0.00903	0.5 ↓↓	0.0013	0.37 ↓↓	0.000215
TSPAN33	MEpink	0.43 ↓↓	0.25 ↓↓	1.84E-05	0.6 ↓	0.00832	0.62 ↓	0.00181
CCL3	MEgreen	0.46 ↓↓	0.25 ↓↓	0.0002371	0.56 ↓	0.0027	0.6 ↓	0.00625
CCL3L1	MEgreen	0.51 ↓	0.11 ↓↓	3.10E-06	0.42 ↓↓	0.000296	0.79	0.149
CCL4	MEgreen	0.51 ↓	0.14 ↓↓	0.0004231	0.2 ↓↓	0.0000307	0.67	0.0684
CXCL9	MEgreen	3.78 ↑↑	32.5 ↑↑	5.00E-07	2.39 ↑↑	0.0174	10.49 ↑↑	0.00448
CXCL10	MEgreen	2.53 ↑↑	8.31 ↑↑	0.00194	3.52 ↑↑	0.00915	4.94 ↑↑	0.000961
LTB	MEgreen	0.40 ↓↓	0.18 ↓↓	0.0001214	0.46 ↓↓	0.0154	0.29 ↓↓	0.00193
MYC	MEgreen	2.34 ↑↑↑	6.77 ↑↑	8.90E-06	4.97 ↑↑	0.000315	1.31	0.15
E2F1	MEblue	1.52 ↑↑	3.1 ↑↑	0.000394	2.53 ↑↑	0.00094	1.15	0.274
EGR2	MEblue	2.09 ↑↑	2.36 ↑↑	0.00203	3.18 ↑↑	0.000003	1.01	0.78
GAS6	MEblue	4.55 ↑↑	16.31 ↑↑	1.01E-05	8.18 ↑↑	0.00255	1.79	0.117
CCL20	not DE	↔	0.98	0.926	1.29	0.351	1.03	0.614

*IL21/CTR

¹: RNA from 13 samples (patient code 1 to 13 in Table 1);

²: RNA from 16 samples (patient code 14 to 29 in Table 1);

³: RNA from 6 healthy donors