|
Escherichia coli
|
|
|
| DH5α |
Host for general cloning |
TranGen Biotech |
| ET12567 (pUZ8002) |
Donor strain for intergeneric conjugation KmR, CmR
|
MacNeil et al. [35] |
|
Streptomyces lomondensis
|
|
| S015 |
Wild-type, lomofungin-producing strain |
Our laboratory |
| DCC601 |
Δlomo10 mutant |
This study |
| DCC602 |
lomo10-complemented strain DCC601 (containing pIB139-lomo10), Aprr
|
This study |
|
Plasmids
|
|
|
| pKC1139 |
E. coli-Streptomyces shuttle vector temperature-sensitive, Aprr
|
Bierman et al. [36] |
| pIB139 |
E. coli-Streptomyces erythraea integrative shuttle vector containing a strong constitutive PermE* promoter, Aprr
|
Wilkinson et al. [37] |
| pMD19-T |
General cloning plasmid, Amr
|
Takara Bio |
| pCC601 |
Left arm of lomo10 subcloned into pMD19-T, Amr
|
This study |
| pCC602 |
Right arm of lomo10 subcloned into pMD19-T, Amr
|
This study |
| pCC603 |
Left and right arms ligated into pMD19-T, Amr
|
This study |
| pKC1139-lomo10
|
Plasmid for in-frame partial deletion of lomo10, left and right arms ligated into pKC1139 at the HindIII/BglII/XbaI sites, Aprr
|
This study |
| pCC604 |
lomo10 ligated into pMD19-T, Amr
|
This study |
| pIB139-lomo10
|
Vector for lomo10 complementation, Aprr
|
This study |
