Table 2. Primers used in this study.
Primers | Sequence (5′–3′) | Enzyme sites |
---|---|---|
lomo10 left arm-For | AAATTTGAATTCTGGATGATCGCGACGATTTC | HindIII |
lomo10 left arm-Rev | AAAAAAAAAAAAATTTAGATCTCATCTCCTGCAGACCCCGAGT | BglII |
lomo10 right arm-For | AAATTTAGATCTGAGTTCGTCAAGGTCAGCTCCC | BglII |
lomo10 right arm-Rev | AAATTTTCTAGACAGTCGGGGAAGCACTTGAG | XbaI |
lomo10-For | ACGCATATGGTGGTGCTCGGGGCCAGCATCG | NdeI |
lomo10-Rev | ACGTCTAGATTCCGATTTCTCAGCGCTGTC | XbaI |
pIB-F [36] | TTGCGCCCGATGCTAGTCG | |
pIB-R [36] | GCACGACAGGTTTCCCGACTG |