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. 2015 Aug 25;11(8):e1005480. doi: 10.1371/journal.pgen.1005480

Fig 1. Expression of a flp-12 neuropeptide reporter is abolished in the SMB neurons of lim-4 mutants.

Fig 1

(A) Schematic drawing of the SMB neurons in C. elegans. Four cell bodies are located in the head (DL: dorsal left, DR: dorsal right, VL: ventral left, VR: ventral right) and their processes run in sublateral cords to the tail. (B) Expression of the flp-12 neuropeptide reporter is abolished specifically in the SMB neurons of lim-4 mutants. GFP expression in ynIs25 integrated strains is observed in the SMB and SDQ(L/R) neurons of wild-type adult (left column) or L1 larval (right column) stage animals while GFP expression in the SMB neurons is not detected in lim-4 null mutant alleles (lsk5, ky403). Faint expression of the flp-12p::gfp reporter in the SAA and AVH/J neurons of L1 larvae is not altered in lim-4 mutants. Anterior is at left in all images. Scale bars: 20 μm. (C) Genomic structure of lim-4 (left) and schematic structure of the LIM domain of LIM-4 (right). The sequence alignment of part of the LIM domain of C. elegans LIM-4 and human LHX6 and LHX8 is shown. Identical residues in at least two proteins are shown in red. Molecular lesions of lim-4 mutant alleles are indicated. *Mutation in ky403 was previously reported [14]. LIM domain and homeodomain are labeled in green and in yellow, respectively.