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. 2015 Aug 25;10(8):e0135986. doi: 10.1371/journal.pone.0135986

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© 2015 Sharma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — Supernatants (diluted 1:4) from cultures of 18 strains of Staphylococcus aureus obtained from the NARSA repository, were tested in a multiplex assay to determine their toxin expression profile. Fluorescence emitted by each Vβ-immobilized bead due to toxin binding, was plotted on bar graphs shown. Toxin(s) secreted by each strain was determined as an increase in fluorescence signal, compared to the background. The error-bars represent standard deviations from two independent experiments. Similar data (but with higher background) were obtained in other experiments where unoccupied sites on biotin-Vβ immobilized-beads were not blocked with excess biotin (S6 Fig).