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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Stem Cells. 2015 Jun 23;33(9):2762–2772. doi: 10.1002/stem.2055

Figure 6. TRBP phosphorylation modulates morphine-induced astrocyte-preferential differentiation of NPCs.

Figure 6

(A) NPCs transfected wild type, SΔA or SΔD TRBP were cultured in complete differentiation medium for 4 d, with or without the treatment of 1 μM morphine. Cells were stained with markers for neurons (Tuj1), astrocytes (GFAP) and with DAPI. Scale bar, 25 μm. Images are representative of four independent experiments with similar results.

(B) Quantification of cells stained with each marker, calculated as the percentage of the total number of cells stained with DAPI. Red: Tuj1; Green: GFAP. *, p<0.05 compared to wild type groups with the same treatment. #, p<0.05 compared to the wild type control.

(C) The mRNA levels of βIII-tubulin and GFAP were determined by qRT-PCR after 4 d of differentiation with indicated treatments. The results were normalized against GAPDH levels. *, p<0.05, **, p<0.01, compared to wild type groups with the same treatment. #, p<0.05 compared to the wild type control. All data represent mean ± SEM of four independent experiments. WT: wild type.