Fig. 3. TQ inhibits TNF-α-induced activation of p38 and JNK, but not ERK or IκBα, in RAFLS.
RA-FLS were pre-treated with TQ (1–5 μM) for 2 hrs, followed by TNF-α (20 ng/ml) stimulation for 30 min. Cell lysates were evaluated for the total and phosphorylated form of p38, JNK, ERK, IκB-α and β-actin by Western blotting (A). Densitometric analysis were done by Image lab 5.1 and values are represented as mean ±SEM of n=4 experiments using different donors (B). ## p<0.01 for NS vs TNF-α; **p<0.01 for TNF-α vs TQ.