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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2015 Jun 29;287(3):299–305. doi: 10.1016/j.taap.2015.06.017

Fig. 4. Involvement of ASK1 in TNF-α stimulated RA fibroblast and their abrogation by TQ.

Fig. 4

RA-FLS were treated with TQ (1–5 μM) for 2 hrs, followed by stimulation with TNF-α (20 ng/ml) for 30 min were lysed and probed for the expression of phospho-ASK1 (Thr845), ASK1, and phospho-c-jun (A). Densitometric analysis normalized with β-actin was done by Image Lab 5.1 and values are the mean ± SEM of experiments performed in four different RA-FLS donors (B). # p<0.05, ## p<0.01 for NS vs TNF-α; *p<0.05, **p<0.01 for TNF-α vs TQ.