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. 2015 Aug 24;230(12):3049–3056. doi: 10.1002/jcp.25041

Figure 4.

Figure 4

Acidosis does not affect ATP release from osteoblasts. Osteoblasts were cultured for up to 14 days at pH 7.4 or 6.9 and ATP release measured at 4, 7, 10 and 14 days. A: Basal ATP release increased with differentiation being eightfold higher in mature, bone forming osteoblasts relative to pre‐osteoblasts. Acidosis did not influence ATP release at any stage of culture. Values are means ± SEM (n = 8–10 replicate wells), ***P < 0.001. B: Osteoblasts cultured for 10 days at pH 7.4 or 6.9 showed similar levels of quinacrine staining. Scale bar = 50 μm.