Table 1.

Primer sequences for RT‐PCR reactions

Gene	Primer sequence (5′–3′)	Product size (bp)	Annealing temperature (°C)
β‐Actin	S – gtt cgc cat gga tga cga t AS – tct ggg tca tct ttt cac gg	332	53
Alpl	S – ctc att tgt gcc aga gaa AS – gtt gta cgt ctt gga gac	238	50
Enpp1	S – gtc agt atg cgt gct aac AS – tgg cac act gaa ctg tag	309	49
Enpp2	S – gcc ctc cgt taa tca tct AS – gca gag aaa gcc act gaa	399	51
Enpp3	S – gca tgc aga gga att gtc AS – tgg gaa cgg tgt atg acc	396	53
NTPdase 1	S – ggg cct atg ggt gga tta ct AS – gta aaa gca cgg gtc ctt ga	332	58
NTPdase 2	S – cca gct atg caa atg aac AS – aac acc cct tca tcc tgt	256	56
NTPdase 3	S – cag cca aac ctt cag atg AS – tgt gcc aca ggt tct tct	356	53
NTPdase 4	S – agg cag ttg tgg aag tca AS – cag aaa tgg agc atc agg	362	53
NTPdase 5	S – tag ctt ggg tta ccg tga AS – ctc ctt cca acc atc ttg	315	53
NTPdase 6	S – ggg atg act gtg ttt cca AS – ttg tca tcc tca gca ggt	322	53
ANK	S – cat cac caa cat agc cat cg AS – aag gca gcg aga tac agg aa	320	55