Fig 1. Study design and treatment schedule for Omniscan injections and deferiprone treatment.

As mentioned in Materials and Methods, after one week of establishment of CKD, one group of mice was administered deferiprone in drinking water (125mg/kg) two days before starting Omniscan injections. Three injections of Omniscan (0.5 mmol/kg, tail IV, 100 μl volume) per week on alternate days were given to two groups of mice (one received deferiprone and the other received Omniscan only) excluding weekends. A total of 10 injections were given. Control mice (one group of CKD and other was sham) animals received 10 injections of an equivalent volume (100 μl) of saline. After 16 weeks at the endpoint, animals were sacrificed and blood and tissues were collected.