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. 2015 Aug 25;10(8):e0136563. doi: 10.1371/journal.pone.0136563

Fig 13. Expression of Omniscan-induced macrophage (CD163) and fibrotic markers (CD34) are iron-regulated, as shown by PBMC in vitro.

Fig 13

Expression of CD163 and fibrotic markers are iron-regulated, as shown by immunofluorescence staining of human PBMC treated with 0.5 mM Omniscan plus deferiprone for 8 days. (A) Representative images show CD163 and CD34 expression by Omniscan, and 25 μM of deferiprone effectively inhibited differentiation of the cells and expression of these markers. (Scale bars 100 μm for all). Western blot and quantitative analysis for western blots are shown in Figs (B) and (C). GAPDH was used as loading control. Values are means ±SD, obtained from 3 separate experiments. Significance of the data was determined by ANOVA, followed by paired-group comparisons. (*p <0.05, for CD163, **p <0.01 for CD34 for Omniscan-treated compared with Omniscan plus deferiprone, **p <0.01, compared with Omniscan alone and control).