Table 1. Primary antibodies used in this study.
IHC—immunohistochemistry, WB—western blot.
| Antibody | Species | Dilution used | Supplier | Catalog number |
|---|---|---|---|---|
| Anti-CD163 | Rabbit (polyclonal) | 1:100 IHC | Santa Cruz Biotechnology, Santa Cruz, CA | SC-33560, Clone M96 |
| Anti-CD163 | Mouse (monoclonal) | 1:1,000 WB | Novus Biologicals, Littleton, CO | NPB2-36494 |
| Anti-CD34 | Mouse (monoclonal) | 1:100 IHC | Santa Cruz Biotechnology | SC-74499 |
| Anti-procollagen-1 | Mouse (monoclonal) | 1:100 IHC | Santa Cruz Biotechnology | Sc-25973 |
| Anti-procollagen-1 | Sheep (monoclonal) | 1:1,000 WB | R&D Systems, Minneapolis, MN | AF6220 |
| Anti-ferroportin/SLC40A1 | Rabbit (polyclonal) | 1:200 IHC | Abcam, Cambridge, MA | ab85370 |
| Anti-ferroportin/SLC40A1 | Rabbit (polyclonal) | 1:1,000 WB | Novus Biologicals | NBP1-21502 |
| Anti-hepcidin | Rabbit (polyclonal) | 1:200 IHC | Abcam | ab81010 |
| Anti-hepcidin | Goat (polyclonal) | 1:1,000 WB | Novus Biologicals | NBP1-59337 |
| GAPDH (HRP conjugated) | Rabbit (monoclonal) | 1:2,000 WB | Cell Signaling Technology, Boston, MA | 8884, Clone D16H11 |