Figure 1.
MSC and ASC mineralization under the effect of exogenous PDGF-BB. MSCs and ASCs were cultured under control (−), control (+), osteogenic (−), and osteogenic (+) conditions for three weeks. Staining after three weeks of culture revealed no mineralization under either control condition (a-h) and an enhancement of mineralization under the presence of PDGF-BB in ASCs (o-p vs. k-l) but not in MSCs (m-n vs. i-j). Quantitative calcium per cell analysis revealed the increased mineralization was on a per-cell basis and the ASC-specific phenomenon was a donor-independent effect over six donors (bottom). Scalebar: 100 μm. *p<0.05.