Fig 5. iNOS and Arg-1 expression in T. cruzi infected cardiac tissue of COX-2^+/+ and COX-2^-/- mice.

(A) Western blot analysis of iNOS and Arg-1 protein in extracts from hearts of COX-2^+/+ and COX-2^-/- from non-infected mice (0 d.p.i.) and at 14 d.p.i. Ponceau staining of the blot is shown as a loading control. Samples for 6 different infected mice are shown. (B) Quantification of iNOS and Arg-1 band areas relative to the Ponceau staining from COX-2^+/+ (dashed black bars) and COX-2^-/- (dashed gray bars) is represented as means ± SEM in arbitrary units. A representative experiment (n = 5) out of two is shown (**p<0.01).