Skip to main content
. 2015 Aug 25;10(8):e0136566. doi: 10.1371/journal.pone.0136566

Fig 1. Generation and validation of Ndr2 knock-out mice.

Fig 1

(A) Genomic structure of the Ndr2 locus in the mouse and targeting vector for Ndr2 inactivation. Exons 1–4, and LoxP sites are indicated. Crosses with Cre-deleter mice removed exon 2, generating an Ndr2 loss-of-function allele. (B) Genotyping of wild-type (+/+), heterozygous (+/-) and homozygous Ndr2 knock-out (-/-) samples by PCR analysis. (C) Western blot analysis of NDR2 protein in colon lysates of wild-type (+/+), heterozygous (+/-) and homozygous Ndr2-deficient (-/-) littermates. (D) Genotype distribution of offspring from Ndr2 heterozygous intercrosses. Genotypes were determined by PCR analysis at weaning.