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. 2015 Aug 25;10(8):e0136566. doi: 10.1371/journal.pone.0136566

Fig 3. NDR kinases are essential for growth, cardiac development and blood vessel remodeling from about embryonic day 8 onward in mouse embryos.

Fig 3

(A, B) Bright field images of wild-type (A) and Ndr1/2-double null (B) littermates at E8.5. Both embryos are of the 6-somite stage. Note that the Ndr1/2-double null somites are small and irregularly shaped. Scale bars correspond to 500μm. (C) Average somite numbers of wild-type and Ndr1/2-double null littermates at E8.5. Data correspond to the analysis of a total of 15 litters and are blotted as box and whisker chart illustrating the distribution of somite numbers per litter and genotype. Average somite numbers are indicated. (D, E) Distribution of Shh (D) and T/brachyury (E) transcripts in wild-type (left) and Ndr1/2-double null littermate embryos (right) at E8.5. Four animals per genotype were analyzed, and all embryos displayed the staining shown in Fig 3D and 3E. (F, G, H) Bright field images of wild-type (F) and Ndr1/2-double null (G, H) littermate embryos at E9.5. 56 Ndr1/2-double null and 163 control embryos at E9.5 were analyzed. White arrow in H points to the pericardial edema. Scale bars correspond to 500μm. (I, J) Bright field images of the yolk sacs of wild-type (I) and Ndr1/2-double null (J) littermate embryos at E9.5. All Ndr1/2-double null yolk sacs (n = 56) displayed defective vascular development as illustrated in Fig 3J.