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. 2015 Aug 25;11(8):e1005478. doi: 10.1371/journal.pgen.1005478

Fig 4. SIC abundance and interference in tel1Δ are similar to wild type.

Fig 4

A) Meiotic chromosomes from wild type and tel1Δ were spread and labeled with antibodies to Zip1 (red) and Zip3-GFP (green). An array of tet operators on the right arm of chromosome IV was identified by coexpression of a tetR-mCherry fusion; the native mCherry signal was used for visualization. Scale bar: 2 μm. B) The number of Zip3-GFP foci on chromosome IV in 204 wild-type and 202 tel1Δ nuclei with full synapsis. Data are pooled from five independent experiments using two independent isolates of each strain. Small but significant decreases in the average number of foci on chromosome IV (7%), and in the total number of foci per cell (3%) were observed for tel1Δ. Individual experiments are shown in S5 Fig. Significance is lost if the most striking experiment is removed. Bars: mean and standard deviation (SD). C) Number of Zip3 foci per cell determined by automated focus finding in ImageJ, using the same images scored in B. Three of five contributing experiments showed a decrease in Zip3 foci, and the difference is statistically significant in two of the three; the other two experiments showed a slight increase in tel1Δ, one of which is statistically significant. Bars: mean and SD. D) Intensity of Zip3 foci. Wild-type and tel1Δ cells marked with arrays of tet operators on either chromosome IV or XIV were mixed on the same slide. The genotype of each cell was identified based on the size of the labeled chromosome. Four different slides were analyzed with equal numbers of wild-type and tel1Δ images from each slide (25 cells of each genotype total). Both labeling schemes (wild type marked on chromosome XIV and tel1Δ marked on chromosome IV, and vice-versa) gave similar results; figure includes data from both sets of strains. Total intensity of each focus is plotted. E) Interference calculated as 1-CoC for Zip3-GFP foci on chromosome IV from 72 wild-type and 76 tel1Δ cells. Error bars: SE. F) Chromosome IV Zip1-stained SC lengths in 209 wild-type and 212 tel1Δ nuclei. Data are pooled from five independent experiments using two independent isolates of each strain; each of the five individual experiments shows a decrease in SC length in tel1Δ, and the difference is statistically significant in two of the five. Individual experiments are shown in S5 Fig. Bars: mean and SD.