Fig 6. Abnormal trophoblast proliferation, differentiation and disorganized placentation in Rac1 conditional-knockout mouse.

(A & B) Increased population of trophoblast giant cells (TGCs) in the placenta of Rac1^d/d uteri. (A) H and E staining of uterine sections from Rac1 ^f/f and Rac1 ^d/d mice on day 10 of pregnancy. TGC and MD indicate trophoblast giant cells and mesometrial decidua, respectively. TGCs are demarcated by dashed lines. Arrows indicate chorionic plate and arrow-heads indicate TGCs. (B) Uterine sections from Rac1 ^f/f and Rac1 ^d/d mice on day 10 of pregnancy were subjected to IF using PL1. (C) Uterine sections from Rac1 ^f/f and Rac1 ^d/d mice on day 10 of pregnancy were subjected to IF using TPBPA and KRT8 antibodies. (D) H and E staining of uterine sections from Rac1 ^f/f and Rac1 ^d/d mice on day 12 of pregnancy. TGC, MD and E indicate trophoblast giant cells, mesometrial decidua, and embryo, respectively.