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. 2015 Aug 25;11(8):e1005458. doi: 10.1371/journal.pgen.1005458

Fig 8. RAC1 regulates secretory function of human endometrial stromal cells.

Fig 8

(A) RAC1 is induced in primary human endometrial stromal cells undergoing in vitro decidualization. Human endometrial stromal cells (HESCs) were subjected to differentiation in response to estrogen, progesterone, and 8-Br-cAMP as described in the Materials and Methods. The expression of RAC1 mRNA was assessed by qPCR. Data represent mean ± SEM from three separate samples and were analyzed by one-way ANOVA with Bonferroni post-test. Asterisks indicate statistically significant differences (*P < 0.05). (B & C) Inhibition of biological activity of RAC1 in HESCs inhibits RAB27B expression and VEGFA secretion in the conditioned media. HESCs were subjected to differentiation in the absence or presence of 25μM InSolution Rac1 Inhibitor II (Z62954982) for eight days. (B) qPCR was performed to monitor the expression of RAC1, RAC2, RHOA, CDC42, VEGFA, and RAB27B, N = 2–3, P< 0.001. (C & D) ELISA was performed to measure VEGFA and IGFBP1 secretion in the conditioned media. VEGFA ELISA data represent mean ± SEM from three separate samples and were analyzed by two-way ANOVA with Bonferroni post-test. Asterisks indicate statistically significant differences (*P < 0.05 and ***P < 0.001). IGFBP1 ELISA data represent mean ± SEM from two separate samples and were analyzed by t-test, P > 0.05.