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. 2015 Aug 25;9(8):e0003853. doi: 10.1371/journal.pntd.0003853

Table 1. Summary of next-generation sequencing data and analysis results.

Condition H3K4Me3 H3K9Ac H3K9Me3 H3K27Me3
cercariae adults schistosomula cercariae adults cercariae adults cercariae adults schistosomula
Number of samples 2 2 2 2 2 2 2 3 3 2
Input reads (average per sample) 34,519,715 6,421,317 49,109,097 54,833,141 43,884,060 53,702,142 43,884,060 40,895,894 33,865,527 46,158,137
Alignment to unique sequences
Aligned exactly 1 time (average per sample) 21,455,086 3,097,077 28,275,831 28,626,785 28,035,327 23,326,061 21,945,541 22,882,234 27,123,469 30,651,658
Aligned reads used for peak-calling (per sample) 2,900,000 2,900,000 2,900,000 23,000,000 23,000,000 21,000,000 21,000,000 17,900,000 17,900,000 17,900,000
Regional peaks identified by peakranger 1.16 (average for condition) 7,746 8,868 7,376 17,116 421 128,518 100,884 145,322 27,544 65,629
Significantly different peaks identified by DiffBind 224 3,844 9,505 8,480
DiffBind result confirmed by visual inspection 124 random sample of 100 peaks for each condition(33–50% confirmed by 2 independent observers)
EpiChIP peaks with signal at FDR 0.01 (per condition) 3,598 3,566 3,954 4,801 4,370 5,024
Alignment to repetitive sequences
Aligned exactly 1 time (average per sample) 1,326,620 242,000 3,805,162 3,091,945 3,881,193 3,612,100 3,999,829 4,554,097
Random sample used for DESeq 220,000 220,000 2,900,000 2,900,000 3,400,000 3,400,000 3,900,000 3,900,000