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. 2015 Aug 25;10(8):e0134205. doi: 10.1371/journal.pone.0134205

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© 2015 Uhlig et al

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Fig 4 — Values are given as numbers and percentages relative to the total number (below pie diagram). A: Type of Euthanasia, Anesthesia*: anesthesia overdose 121 (25%), anesthesia overdose + transcardial perfusion 135 (28%), anesthesia overdose + decapitation 38 (8%), anesthesia overdose + removal of vital organs 17 (4%), anesthesia overdose + exsanguination 37 (8%), number and percentage relative to total, respectively. B: Euthanasia drugs. Inhalational: isoflurane 47 (13%), carbon dioxide 29 (8%), number and percentage relative to total, respectively. #: urethane is not recommended as sole method for euthanasia of small laboratory mammals by the guidelines of the American Veterinary Medical Association (AVMA) guidelines for the euthanasia of animals [22] due to its slow onset of action. Other: drugs with <2% occurrence. n/s: not specified.