Figure 3. Effect of Cav-1 expression in prostate cancer cells on LEC chemotaxis and chemokinesis.

(A) LEC ability to migrate randomly in two-dimension was tested using a wound healing assay during which cells were incubated in various prostate cancer cell-conditioned media for 6 h. Results are reported as percent of wound closure and shown as mean ± S.E.M. (n=3 separate experiments), *p<0.05. (B) LEC directional migration toward conditioned media from each prostate cancer cell line was assessed using the modified Boyden chamber assay. Results are reported as percent of the migration to conditioned medium of PCa control cells and shown as mean ± S.E.M. (n=3-5 separate experiments), **p<0.01.