Table 3.
Primers and probes for real-time quantitative PCR of EBV
Assay | Sequence | References |
---|---|---|
LMP1 | ||
Sense | 5′-CCC TTT GTA TAC TCC TAC TGA TGA TCA C | Kubota et al. (2008) |
Antisense | 5′-ACC CGA AGA TGA ACA GCA CAA T | Kubota et al. (2008) |
Probe | 5′-CTC ATC GCT CTC TGG AAT TTG CAC GG | Kubota et al. (2008) |
LMP2 | ||
Sense | 5′-AGC TGT AAC TGT GGT TTC CAT GAC | |
Antisense | 5′-GCC CCC TGG CGA AGA G | |
Probe | 5′-CTG CTG CTA CTG GCT TTC GTC CTC TGG | |
EBNA1 | ||
Sense | 5′-TAC AGG ACC TGG AAA TGG CC | Ryan et al. (2004) |
Antisense | 5′-TCT TTG AGG TCC ACT GCC G | Ryan et al. (2004) |
Probe | 5′-AGG GAG ACA CAT CTG GAC CAG AAG GC | |
EBNA2 | ||
Sense | 5′-TCT TGC GTT ACA TGG GGG AC | |
Antisense | 5′-CCT GGT AGG GAT TCG AGG GA | |
Probe | 5′-AAT TGT TGA CAC GGA TAG TCT TGG | |
BZLF1 | ||
Sense | 5′-AAA TTT AAG AGA TCC TCG TGT AAA ACA TC | Ryan et al. (2004) |
Antisense | 5′-CGC CTC CTG TTG AAG CAG AT | Ryan et al. (2004) |
Probe | 5′-ATA ATG GAG TCA ACA TCC AGG CTT GGG C | |
EA-D | ||
Sense | 5′-CGT GCC AAT CTT GAG GTT TT | |
Antisense | 5′-CAC CCG GGG ACT TTT ATC TT | |
Probe | 5′-TTT ATT TAA CCA CGC CTC CG | |
β-Actin | ||
Sense | 5′-CCT GGC ACC CAG CAC AAT G | |
Antisense | 5′-GCC GAT CCA CAC GGA GTA CT | |
Probe | 5′-ATC AAG ATC ATT GCT CCT CCT GAG CGC |