Fig. 4.

Effect of RAPA on 4-HNE-induced loss of synaptophysin protein in SH-SY5Y cells. (a) experiment design. RAPA at different doses and corresponding volumes of Vehicle was added to SH-SY5Y cells. Cells were then treated with 4-HNE (15 µM) 19 h later for 5 h in the same medium before protein extraction. (b) representative immunoblot. Each well of 4–12% Criterion gel was loaded with 100 µg cell lysate. (c) quantification of immunoblot by Odyssey software. Each group contained nine replicates. Data were analyzed by a two-way ANOVA followed by Bonferonni post hoc n=9, p<0.05, **p<0.01.