Fig. 4.

Co-expression of CB₁R and CB₂R mRNA in pallidothalamic neurons in control, parkinsonian and dyskinetic monkeys. Immunofluorescent detection of transported CTB combined with dual fluorescent in situ hybridization for the detection of CB₁R and CB₂R mRNA. All pallidothalamic projecting neurons (green channel) co-expressed CB₁R (red channel) and CB₂R (blue channel) mRNA. CB₂R mRNA was expressed at lower levels than CB₁R mRNA across all experimental conditions. Most importantly, there was a marked reduction in expression levels for both CB₁R and CB₂R mRNA transcripts in the dyskinetic state. Scale bar is 50 μm for all panels