TABLE 2.
Limiting Dilution Analysis
| Cell number implanted | Vehicle-treatment | Calcitriol-treatment |
|---|---|---|
| 500 | 5/6 | 4/5 |
| 100 | 5/5 | 1/5 |
| 50 | 4/5 | 1/5 |
|
| ||
| TIC frequency (p < 0.01) | 1/98 (1/66 – 1/147) | 1/320 (1/207 – 1/495) |
Number of tumors formed/number of injections
Limiting dilution analysis of purified CD49fhighEpCamlow TIC-enriched cells from MMTV-Wnt1 mammary tumors. TICs were cultured in the presence of calcitriol (1 nM) or vehicle (ethanol). After 2 weeks, breast tumor spheroids were dissociated into single cells, and varying numbers of viable tumor cells were orthotopically implanted into the mammary fat pad and assayed for tumor initiation. TIC frequencies and p-value were calculated using L-Calc. B,