(a).

Preparations	Theoretical maximal bound activity (U)	Unbound activity (U)	Bound (actual) activity (U)	Immobilization efficiency∗	Activity retention#	Fold enrichment†
Entrapped	114.67 ± 4.15	98.55 ± 3.06	87.4 ± 3.63	76 ± 2.24	53 ± 3.55	41 ± 3.00
Cross-linked	210.14 ± 6.31	3.08 ± 0.35	194.75 ± 6.53	92 ± 3.17	99 ± 3.78	91 ± 7.00

^∗Immobilization efficiency = (actual bound activity/maximum theoretical bound activity) × 100.

^#Activity retention = (maximum theoretical bound activity/total activity employed for immobilization) × 100.

^†Fold enrichment = (actual bound activity/total activity employed for immobilization) × 100.

Note: immobilization effectiveness factor can be calculated from immobilization efficiency by dividing it with 100. Immobilization yield can be calculated from fold enrichment by dividing it with 100.

Enzyme units used for the immobilization: 213.22 UmL⁻¹.