(b).
Residual activity (%) | |||
---|---|---|---|
Free enzyme | Entrapped enzyme | Cross-linked | |
Metal ions (5 mM) | |||
NaCl | 124.9 ± 5.66 | 73.6 ± 2.92 | 74.2 ± 3.21 |
KCl | 166.6 ± 6.84 | 92.1 ± 4.25 | 96.9 ± 4.47 |
MgCl2 | 93.8 ± 3.62 | 57.5 ± 2.43 | 63.5 ± 3.02 |
CaCl2 | 84.6 ± 2.68 | 87.6 ± 3.73 | 80.6 ± 3.45 |
CoCl2 | 33.4 ± 1.32 | 41.1 ± 1.64 | 38.0 ± 1.12 |
HgCl2 | 9.8 ± 1.02 | 37.04 ± 2.46 | 39.9 ± 1.82 |
CdCl2 | 15.9 ± 1.68 | 36.8 ± 2.37 | 35.9 ± 2.54 |
NiCl2 | 59.9 ± 5.23 | 47.4 ± 4.02 | 49.8 ± 4.17 |
Lead acetate | 35.2 ± 2.63 | 73.2 ± 3.61 | 78.9 ± 4.23 |
Chemicals (1 mM) | |||
PMSF∗ | 97.3 ± 5.62 | 104.9 ± 6.10 | 127.3 ± 6.55 |
PHMB# | 4.7 ± 0.58 | 34.7 ± 2.81 | 47.6 ± 3.19 |
DTNB† | 5.0 ± 0.95 | 29.4 ± 1.66 | 53.2 ± 2.58 |
EDTA‡ | 90.8 ± 6.24 | 82.71 ± 5.77 | 84.5 ± 5.29 |
∗PMSF: phenylmethanesulfonyl fluoride.
#PHMB: para-hydroxymercurybenzoate.
†DTNB: 5,5′-dithiobis-(2-nitrobenzoic acid).
‡EDTA: ethylenediaminetetraacetic acid.