Table 2. Specific activities of catechol 1,2-dioxygenase in crude cell extracts from different engineered strains with wild-type and mutated catA.

Strains*	Specific Activity (U/mg)**
WT	2.489 ± 0.112
G72P	0.006 ± 0.002
G72A	15.828 ± 0.491
G72V	0.004 ± 0.001
L73F	6.425 ± 0.301
L73M	0.0652 ± 0.003
P76A	25.2629 ± 0.626
P76G	0.043 ± 0.002
P76V	0.005 ± 0.002
L73F/P76A	14.019 ± 0.549

^*WT indicates E. coli AB2834 containing pKD2.834A and pKD2.292 with wild-type catA. Other strains contain different mutated catAs.

^**1U = 1 μmol ccMA produced per minute at 24 °C. Errors (standard deviations) were calculated based on at least three independent experiments.