. 2015 Aug 26;5:13435. doi: 10.1038/srep13435

Table 3. Enzymatic kinetic parameters of wild-type and mutated CatAs after purification against the substrate catechol^*.

Enzymes	K_m(μM)	k_cat(S⁻¹)
WT	7.88 ± 1.239	7.86 ± 0.248
L73F	151.09 ± 10.299	5.65 ± 0.163
P76A	65.91 ± 7.753	46.56 ± 0.201
L73F P76A	45.96 ± 7.691	39.50 ± 0.221

^*WT indicates wild-type CatA. Others are mutated proteins. Errors (standard deviations) were calculated based on at least three independent experiments.

Table 3. Enzymatic kinetic parameters of wild-type and mutated CatAs after purification against the substrate catechol*.

Table 3. Enzymatic kinetic parameters of wild-type and mutated CatAs after purification against the substrate catechol^*.