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. 2015 Aug 26;6:669. doi: 10.3389/fpls.2015.00669

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Copyright © 2015 Song, Wang, Zhang, Li, Li, Guo, Niu, Wang and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Enrichment assessment of various subcellular membranous components. (A) Wavelength scanning of reaction system of H⁺-ATPase activity determination at a wavelength of 400–900 nm. (B) Enzymatic characterization of the membrane fraction. Relative H⁺-ATPase activity was determined by measurement of membrane-specific H⁺-ATPase activity compared to total ATPase activity. MF, membrane fraction; CH, crude homogenate; VM, vacuole membrane; PM, plasma membrane; MM, mitochondria membrane. Data are means ± SD of three independent experiments (biological replicates).