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. 2015 Aug 26;9:333. doi: 10.3389/fncel.2015.00333

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Copyright © 2015 Iseppon, Napolitano, Torre and Cojoc.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optical manipulation and FRET imaging setup. The IR laser beam (1064 nm) is aligned to the UV laser micro-dissection beam (355 nm) and then both are directed into the pupil of the microscope objective (60X - 1.2 NA). The sample is illuminated both by a white light source and a Hg fluorescence lamp. The light coming from the Donor and the Acceptor fluorophores in the sample is separated by a dual emission image splitter and the two images are formed on the two halves of the sCMOS camera sensor.