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. 2015 Aug 26;9:333. doi: 10.3389/fncel.2015.00333

Figure 3.

Figure 3

Spontaneous Cdc42 dynamics in NG108-15 cells. (A) Ratiometric FRET live imaging of a representative Cdc42-FRET neuroblastoma cell (n = six experiments). Consecutive frames were taken every second (left: generation of a ratiometric image) for 15 min. Insets on the right show a time series (1 frame every 3 min) of the magnified region denoted by the white box in the left image. Arrows indicate protrusion and retraction directions. The red line shows the initial GC profile. Scale Bar: 20 μm. (B) Quantification of mean Cdc42 activity (FRET ratio) in 30 stalling, protruding and retracting sections along the cell edge from Cdc42 neuroblastoma cells from two experiments. *p < 0.05, ***p < 0.001 using ANOVA test (C,D) Montage images showing the dynamics of Cdc42 activation in a protruding region of the cell. (C) Images showing the region of interest selected for the montage (white boxes). (D) Montage images highlighting hotspots of Cdc42 in the protruding region. Intensity scale on the right in (D) applies to (A,C,D).