Table 1. The effect of Ganoderma triterpenoids on the activity of NAs.
| Compound | Inhibition rate (%)* |
|||||
|---|---|---|---|---|---|---|
| NA(H1N1,09) | NA(H1N1, N295S) | NA(H3N2, E119V) | NA (H5N1) | NA(H7N9) | ||
| 1.Ganoderic acid T-Q | 81.7 ± 1.7 | 62.7 ± 1.7 | 55.4 ± 4.1 | 94.4 ± 0.4 | 27.4 ± 1.2 | |
| 2.Ganoderic acid TR | 87.4 ± 1.6 | 57.7 ± 0.9 | 59.2 ± 1.9 | 96.5 ± 0.3 | 24.0 ± 1.8 | |
| 3.Ganoderic acid T-N | 72.0 ± 5.5 | 27.5 ± 1.4 | 43.6 ± 1.2 | 91.1 ± 0.8 | 25.6 ± 1.6 | |
| 4.Ganoderic acid Sz | 60.1 ± 4.8 | 29.3 ± 5.1 | 22.5 ± 0.3 | 0.0 | 17.8 ± 4.0 | |
| 5.Ganoderic acid S | 69.4 ± 2.3 | 34.1 ± 2.2 | 44.9 ± 3.2 | 86.7 ± 0.5 | 31.9 ± 2.3 | |
| 6.Ganoderic acid Y | 63.0 ± 2.5 | 16.9 ± 2.8 | 23.2 ± 3.4 | 78.2 ± 3.2 | 17.8 ± 1.5 | |
| 7.Ganoderic acid A | 5.0 ± 5.4 | 15.3 ± 1.8 | 32.1 ± 2.7 | 31.4 ± 7.3 | 6.3 ± 2.2 | |
| 8.Ganoderenic acid A | 35.3 ± 3.7 | 12.4 ± 1.8 | 29.1 ± 2.3 | 35.9 ± 7.7 | 23.3 ± 3.1 | |
| 9.Ganoderic acid C2 | 32.4 ± 4.5 | 13.7 ± 2.4 | 45.4 ± 1.1 | 60.8 ± 4.3 | 27.5 ± 2.3 | |
| 10.Ganoderic acid AM1 | 28.3 ± 6.2 | 9.3 ± 1.6 | 31.3 ± 3.2 | 75.2 ± 2.3 | 14.1 ± 5.1 | |
| 11.Ganoderic acid K | 28.8 ± 2.0 | 14.2 ± 2.9 | 33.5 ± 2.4 | 57.7 ± 10.9 | 28.5 ± 1.9 | |
| 12.Ganoderenic acid H | 58.0 ± 3.0 | 12.8 ± 5.1 | 38.6 ± 2.3 | 77.7 ± 3.9 | 38.6 ± 6.2 | |
| 13.Ganoderic acid H | 35.0 ± 4.5 | 16.9 ± 2.4 | 27.9 ± 1.5 | 64.1 ± 4.5 | 27.2 ± 3.2 | |
| 14.Ganoderic acid B | 6.9 ± 6.8 | 12.8 ± 1.4 | 38.7 ± 1.6 | 3.0 ± 1.0 | 5.5 ± 0.4 | |
| 15.Ganoderenic acid F | 53.1 ± 1.6 | 24.4 ± 4.4 | 24.2 ± 3.9 | 62.8 ± 4.0 | 34.2 ± 4.7 | |
| 16.Ganoderenic acid C | 27.9 ± 0.3 | 13.2 ± 2.2 | 37.5 ± 2.4 | 30.6 ± 4.5 | 27.2 ± 0.5 | |
| 17.Ganoderenic acid D | 50.4 ± 3.0 | 13.8 ± 3.1 | 32.2 ± 3.4 | 74.2 ± 2.2 | 35.9 ± 2.8 | |
| 18.Ganoderic acid C6 | 17.8 ± 1.2 | 17.6 ± 3.5 | 20.7 ± 0.5 | 15.6 ± 22.3 | 25.7 ± 1.3 | |
| 19.Ganoderic acid C1 | 21.1 ± 4.1 | 14.7 ± 3.9 | 32.0 ± 3.4 | 43.3 ± 6.1 | 26.2 ± 4.1 | |
| 20.Ganoderic acid DM | 50.4 ± 1.5 | 19.0 ± 2.4 | 28.8 ± 0.5 | 55.2 ± 0.7 | 29.4 ± 3.8 | |
| 21.Ganolucidic acid A | 19.4 ± 2.7 | 21.0 ± 4.2 | 22.9 ± 3.8 | 49.8 ± 7.7 | 16.7 ± 3.4 | |
| 22.Ganoderic acid Zeta | 35.9 ± 5.3 | 25.5 ± 4.9 | 18.7 ± 4.5 | 0.0 | 17.7 ± 2.4 | |
| 23.Ganoderic acid LM2 | 40.9 ± 6.2 | 15.4 ± 0.6 | 12.9 ± 1.0 | 67.1 ± 5.2 | 12.7 ± 9.6 | |
| 24.Ganoderic acid F | 12.9 ± 11.4 | 17.6 ± 2.0 | 19.9 ± 2.6 | 48.6 ± 7.4 | 14.8 ± 2.6 | |
| 25.Ganoderol A | 48.3 ± 2.2 | 22.6 ± 1.8 | 18.2 ± 2.7 | 77.1 ± 0.4 | 15.9 ± 3.7 | |
| 26.Ganoderol B | 51.0 ± 2.6 | 19.9 ± 1.1 | 19.1 ± 6.1 | 71.6 ± 2.1 | 21.8 ± 3.5 | |
| 27.Ganoderiol F | 52.9 ± 2.0 | 9.5 ± 4.5 | 16.8 ± 1.2 | 33.6 ± 12.1 | 29.5 ± 4.3 | |
| 28.Ganodermanondiol | 53.1 ± 2.5 | 19.0 ± 4.2 | 34.2 ± 0.9 | 87.9 ± 1.8 | 24.3 ± 1.7 | |
| 29.Ganodermanontriol | 35.3 ± 5.2 | 7.9 ± 3.2 | 12.2 ± 3.4 | 60.5 ± 6.8 | 10.3 ± 3.5 | |
| 30.Lucialdehyde A | 57.6 ± 1.7 | 5.8 ± 2.4 | 25.9 ± 8.1 | 67.1 ± 5.7 | 24.5 ± 1.6 | |
| 31.Lucialdehyde B | 51.4 ± 2.5 | 11.2 ± 1.3 | 43.3 ± 1.0 | 86.7 ± 0.8 | 30.4 ± 3.5 | |
*Inhibition rates were calculated from independent NA inhibition assays (n = 3) that used 200 μM of each compound and different NA subtypes. They are expressed as means ± standard deviation. The values showed in boldface are the top three inhibitors against each NA subtype. The difference between NA subtypes were determined by the paired Wilcoxon signed-rank test (n = 31): NA (H1N1, 09) vs. NA (H3N2, E119V), P = 0.002; NA (H1N1, 09) vs. NA (H7N9), P = 0.00001; NA (H5N1) vs. NA (H3N2, E119V), P = 0.00003; NA (H5N1) vs. NA (H7N9), P = 0.000008; NA (H1N1, N295S) vs. NA (H7N9), P0= 0.085; NA (H1N1, 09) vs. NA (H1N1, N295S), P = 0.000005.