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. 2015 Jul 28;7(8):2822–2834. doi: 10.3390/toxins7082822

Figure 4.

Figure 4

Effect of cantharidin on reactive oxygen species: (A) Original histogram of 2′,7′-dichlorodihydrofluorescein diacetate (DCFDA) fluorescence in erythrocytes following exposure for 48 h to Ringer solution without (grey shadow) or with (black line) the presence of 50 μg/mL cantharidin or, for comparison, following a one hour exposure to 100 μM tert-butyl-hydroperoxide (tBOOH, light grey line). (B) Arithmetic means ± SEM (n = 4) of the erythrocyte DCFDA fluorescence following incubation for 48 h to Ringer solution without (control, white bar) or with presence of 50 μg/mL cantharidin (black bar) or for one hour with presence of 100 μM tBOOH (black bar). *** (p < 0.001) indicates significant difference from the absence of cantharidin and tBOOH (ANOVA).