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. 2015 Aug 5;7(8):2985–2999. doi: 10.3390/toxins7082985

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Aflatoxin production of select A. flavus isolates under H₂O₂-induced oxidative stress in toxin-conducive yeast extract-sucrose (YES) and non-conducive yeast extract-peptone (YEP) media visualized using thin layer chromatography (TLC). (A) Isolate NRRL3357 was cultured in YES media containing 15% sucrose and supplemented with H₂O₂ ranging from 0 (control check) to 10 mM. Increasing visible fluorescence over the concentration gradient indicates elevated levels of aflatoxin B₁₊₂. (B) Isolates AF13 and NRRL3357 were cultured on YES and YEP media containing reduced carbon source concentrations (1%). Elevated aflatoxin production was observed in response to oxidative stress in 1% YES media (compared to “0” control) while no aflatoxin production was observed in 1% YEP medium. An aflatoxin B₁ standard was included as a reference.