Fig. 4.

CaSR regulates growth kinetics of HT29 and Caco2-15 cells. Stably transfected cells were counted 24, 48 and 72 h post-seeding. Stable over-expression of CaSR-WT was associated with reduced proliferation rates. Overexpression of CaSR-DN however, was associated with increased growth rates. Cells stably transfected with the empty vector (EMP) were used as controls. Data were normalized to number of viable cells 24 h post-seeding. Bars represent means ± SEM of 3–5 independent experiments. Statistical significance was determined by ANOVA followed by Tukey's post-test. Asterisks above bars indicate significant changes within groups (compared with EMP cells), *p < 0.05, ***p < 0.001.