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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: Science. 2015 Apr 2;348(6236):803–808. doi: 10.1126/science.aaa3828

Fig. 4. Vaccination promotes a diverse neoantigen-specific T cell repertoire.

Fig. 4

(A) Summary of TCRβ clonotypes identified, using neoantigen-specific TCRβ CDR3 reference libraries (see Tables S6-S10), in CD8+ T cell populations isolated from PBMC obtained before and after vaccination. Each symbol represents a unique TCRβ sequence and its frequency (%) in pre- and post-vaccine samples. Wilcoxon-signed rank test was performed and p values indicated in figure. (B) TCRβ CDR3 sequence of clonotypes (Tables S6-S10) identified in pre- (black bars) and post- (white bars) vaccine CD8+ T cell populations for neoantigens TKT R438W (pre=5, post=84 clonotypes); SEC24A P469L (pre=9, post=61) and EXOC8 Q656P (pre=2, post =12). Frequency of each unique clonotype is reported as percentage of total read counts.