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. 2015 Aug 6;10(1):1–23. doi: 10.1016/j.molonc.2015.07.005

Figure 6.

Figure 6

Co‐localization of rLOX‐PP‐Atto565 and CTxB‐Alexa647 in various cell lines. Cells were incubated with CtxB‐Alexa647 (652/668 nm‐magenta) and rLOX‐PP‐Atto565 (563/592 nm‐red) for 3 h before imaging. Confocal microscope pictures were formatted in split Z‐stacks on the left and merged on the right for each cell line. PWR‐1E (A), DU145 (B), PC3 (C), SCC9 (D) and MDA‐MD‐231(E) cell lines were listed; (DIC), dichromatic image of cells. Various levels of co‐localization of rLOX‐PP‐Atto565 with CTxB‐Alexa647 in PWR‐1E, PC3, and MDA‐MC‐231 cell lines confirm that rLOX‐PP‐Atto565 enters the cell through a caveola‐mediated pathway.