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. 2015 Aug 6;10(1):1–23. doi: 10.1016/j.molonc.2015.07.005

Figure 13.

Figure 13

Removal of (His) 6 ‐tag increases rLOX‐PP‐Atto565 uptake, while pretreatment with rLOX‐PP decreases its uptake. In (A), DU145 cells and PC3 cells were treated with either rLOX‐PP‐Atto565 with His‐tag (gray bars) or without (His)6‐tag (white bars), and uptake levels quantified as a function of time by flow cytometry; n = 3, *, p < 0.005. (B) Cells were pre‐treated with unlabeled rLOX‐PP, followed by rLOX‐PP‐Atto56, and uptake of rLOX‐PP‐Atto565 determined; data are means ± SD, n = 3, *, two‐tailed p < 0.05 (Except in MDA‐MB‐231). (C) A representative confocal image showed that removal of (His)6‐tag increased rLOX‐PP‐Atto565 uptake in PC3 cells at 3 h (images were collected with EC‐Plan Neofluar 40x/1.30 oil DIC M27 objective).