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. 2015 Aug 27;10:149. doi: 10.1186/s13000-015-0382-1

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© Feng et al. 2015

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Down-regulation of CRT induces the cell apoptosis in HCC cells. a. SMMC7721 cells were transfected with CRT siRNA and negative control siRNA for 36 h. The apoptosis was examined by Annexin V/FITC and propidium iodide staining method using flow cytometry. SMMC7721 without any transfection served as a negative control. SMMC7721 apoptotic rates were quantified. Each bar of the histogram represents the quantified data shown as the mean ± SD of at least three independent experiments. b. HepG2 cells were transfected with CRT siRNA and negative control siRNA for 36 h. The apoptosis was examined by Annexin V/FITC and propidium iodide staining method using flow cytometry. HepG2 without any transfection served as a negative control. HepG2 apoptotic rates were quantified. Each bar of the histogram represents the quantified data shown as the mean ± SD of at least three independent experiments. Figures shown are representatives of at least 3 independent experiments. Statistical differences were compared with the siCtrl and NC (*P <0.05)